Abstract:[Objective]The induction and anti-insect effects of exogenous methyl jasmonate to larch were clarified, which will provide the theoretical basis for the ecological control of larch defoliator, through the study on the effects of exogenous methyl jasmonate (MeJA) on host selection behavior of Dendrolimus superans. [Method]Dendrolimus superans, two-year-old seedlings of L. olgensis sprayed with different concentrations of MeJA, and the effects of these treatments on behavioral responses of the moth were tested by cages and Y-tube olfactometers. [Result] MeJA-treated seedlings repelled the female moth and made the moth lay lots of eggs on control seedlings. Compared with control, the oviposition percent of the female moth on 1, 5 or 10 mmol/L MeJA-treated seedlings decreased by 47.21%--77.03%, 43.18%--55.46% or 71.80%--80.08%; then the olfactory response ratio of the female moth on 1, 5 or 10 mmol/L MeJA-treated seedlings reduced by 49.24%, 59.71% or 43.68%--61.24%, and the higher concentration MeJA was used to treat the seedlings, the more obvious effect was observed on the behavior responses of the moth. [Conclusion] The MeJA-treated L. olgensis seedlings distinctly affect the olfactory and oviposition choice behavior of the female moth. While the stress responses of seedlings are relevant to the concentrations of MeJA, and the induced function of MeJA can last for 3--5 days.
孟昭军, 林健, 王琪, 鲁艺芳, 王雷, 严善春. 落叶松毛虫对茉莉酸甲酯处理长白落叶松苗的行为反应[J]. 北京林业大学学报, 2018, 40(12): 60-67.
Meng Zhaojun, Lin Jian, Wang Qi, Lu Yifang, Wang Lei, Yan Shanchun. Behavioral responses of Dendrolimus superans to Larix olgensis seedlings treated with methyl jasmonate. Journal of Beijing Forestry University, 2018, 40(12): 60-67.
[1]张华峰,陈顺立,康文通,等. 桉树枝瘿姬小蜂危害及不同诱导方式对桉树挥发物释放的影响[J]. 福建林学院学报,2013,33(2):170--175. Zhang H F, Chen S L, Kang W T, et al. Effects of different inductions on volatiles of Eucalyptus grandis damaged by Leptocybe invasa Fisher et La Salle[J]. Journal of Fujian College of Forestry, 2013, 33(2):170--175. [2]Ghimire R P, Kivimäenpää M, Blomqvist M, et al. Effect of bark beetle (Ips typographus L.) attack on bark VOC emissions of Norway spruce (Picea abies Karst.) trees[J]. Atmospheric Environment, 2016, 126:145--152. [3]Thaler J S, Stout M J, Karban R, et al. Jasmonate-mediated induced plant resistance affects a community of herbivores[J]. Ecological Entomology, 2001, 26:312--324. [4]Bruinsma M, Van Dam N M, Van Loon J J A, et al. Jasmonic acid-induced changes in Brassica oleracea affect oviposition preference of two specialist herbivores[J]. Journal of Chemical Ecology, 2007, 33:655--668. [5]赵成华,阎云花. 马尾松针叶中的挥发物质对马尾松毛虫产卵行为的影响[J]. 林业科学,2003,39(6): 91--93. Zhao C H, Yan Y H. Oviposition behaviour of the pine caterpillar moth, Dendrolimus punctatus influenced by needle volitales of Pinus massoniana[J]. Scientia Silvae Sinicae, 2003, 39(6): 91--93. [6]李继泉,樊慧,金幼菊. 光肩星天牛对损伤后复叶槭植株的行为反应[J]. 北京林业大学学报,2003,25(5): 42--46. Li J Q, Fan H, Jin Y J. Behavior response of Anoplophora glabripennis to the mechanical-wounded and herbivore-fed ashleaf maples[J]. Journal of Beijing Forestry University, 2003, 25(5): 42--46. [7]Heijari J, Nerg A M, Kainulainen P, et al. Application of methyl jasmonate reduces growth but increases chemical defence and resistance against Hylobius abietis in Scots pine seedlings[J]. Entomologia Experimentalis et Applicata, 2005, 115: 117--124. [8]Erbilgin N, Krokene P, Christiansen E, et al. Exogenous application of methyl jasmonate elicits defenses in Norway spruce (Picea abies) and reduces host colonization by the bark beetle Ips typographus[J]. Oecologia, 2006, 148: 426--436. [9]Zas R, Bjöklund N, Nordlander G, et al. Exploiting jasmonate-induced responses for field protection of conifer seedlings against a major forest pest, Hylobius abietis[J]. Forest Ecology and Management, 2014, 313: 212--223. [10]严善春,胡隐月,孙江华,等. 落叶松挥发性物质与球果花蝇危害的关系[J]. 林业科学,1999,35(3):58--62. Yan S C, Hu Y Y, Sun J H, et al. Larch cone volatile profile and its damage by cone fly (Strobilomyia spp.) in northeastern China[J]. Scientia Silvae Sinicae, 1999, 35(3): 58--62. [11]严善春,刘英胜,王琪,等. 落叶松毛虫对兴安落叶松6种挥发物的触角电位反应[J]. 林业科学,2007,43(7):55--60. Yan S C, Liu Y S, Wang Q, et al. Electroantennogram responses of Dendrolimus superans to six volatiles of Larix gmelinii[J]. Scientia Silvae Sinicae, 2007, 43(7): 55--60. [12]孟昭军,严善春,徐伟,等.长白落叶松8个家系挥发性化合物的比较分析[J]. 林业科学,2008,44(6):91--96. Meng Z J, Yan S C, Xu W, et al. Comparative analysis of volatile profiles of eight families of Larix olgensis[J]. Scientia Silvae Sinicae, 2008, 44(6): 91--96. [13]严善春,徐伟,袁红娥,等. 不同诱导因子对落叶松毛虫嗅觉和产卵选择的影响[J]. 应用生态学报,2007,18(7):1583--1588. Yan S C, Xu W, Yuan H E, et al. Effects of different elicitors on olfactory response and oviposition selection of Dendrolimus superans (Butler) [J]. Chinese Journal of Applied Ecology, 2007, 18(7): 1583--1588. [14]Meng Z J, Yan S C, Yang C P, et al. Behavioural responses of Dendrolimus superans and Anastatus japonicus to chemical defences induced by application of jasmonic acid on larch seedlings[J]. Scandinavian Journal of Forest Research, 2011, 26: 53--60. [15]Lu Y B, Liu S S, Liu Y Q, et al. Contrary effects of jasmonate treatment of two closely related plant species on attraction of and oviposition by a specialist herbivore[J]. Ecology Letters, 2004, 7: 337--345. [16]Heijari J, Nerg A M, Kainulainen P, et al. Long-term effects of exogenous methyl jasmonate application on Scots pine (Pinus sylvestris) needle chemical defence and diprionid sawfly performance[J]. Entomologia Experimentalis et Applicata, 2008, 128: 162--171. [17]Gols R, Posthumus M A, Dicke M. Jasmonic acid induces the production of gerbera volatiles that attract the biological control agent Phytoseiulus persimilis[J]. Entomologia Experimentalis et Applicata, 1999, 93: 77--86 [18]吴俊民,礼波宁,刘广平,等. 混交林中落叶松挥发性物质对水曲柳生长的影响[J]. 东北林业大学学报,2000,28(1):25--28. Wu J M, Li B N, Liu G P, et al. Effect of volatile substance of larch on the growth of ash in mixed forest plantation[J]. Journal of Northeast Forestry University, 2000, 28(1): 25--28. [19]Moreira X, Lundborg L, Zas R, et al. Inducibility of chemical defences by two chewing insect herbivores in pine trees is specific to targeted plant tissue, particular herbivore and defensive trait[J]. Phytochemistry, 2013, 94: 113--122. [20]孙晓玲,董文霞,蔡晓明,等. 外用不同浓度茉莉酸甲酯诱导的茶树挥发物的种类和时序变化[J]. 应用昆虫学报,2016,53(3):499--506. Sun X L, Dong W X, Cai X M, et al. Variation in tea-plant volatiles induced by exogenous application of different concentrations of methyl jasmonate[J]. Chinese Journal of Applied Entomology, 2016, 53(3): 499--506. [21]Arce C C M, Machado R A R, Ribas N S, et al. Nematode root herbivory in tomato increases leaf defenses and reduces leaf miner oviposition and performance[J]. Journal of Chemical Ecology, 2017, 43:120--128. [22]Disi J O, Zebelo S, Ngumbi E, et al. cis-Jasmone primes defense pathways in tomato via emission of volatile organic compounds and regulation of genes with consequences for Spodoptera exigua oviposition[J]. Arthropod-Plant Interactions, 2017, 11: 591--602. [23]Sobhy I S, Woodcock C M, Powers S J, et al. cis-Jasmone elicits aphid-induced stress signalling in potatoes[J]. Journal of Chemical Ecology, 2017, 43: 39--52. [24]Moreira X, Sampedroa L, Zasb R. Defensive responses of Pinus pinaster seedlings to exogenous application of methyl jasmonate: concentration effect and systemic response[J]. Environmental and Experimental Botany, 2009, 67: 94--100. [25]Graves A D, Holsten E H, Ascerno M E, et al. Protection of spruce from colonization by the bark beetle, Ips perturbatus, in Alaska[J]. Forest Ecology and Management, 2008, 256: 1825--1839. [26]Baldwin I T. Methyl jasmonate-induced nicotine production in Nicotiana attenuata: inducing defence in the field without wounding[J]. Entomologia Experimentalis et Applicata, 1996, 80: 213--220. [27]Giacomuzzi V, Cappellin L, Khomenko I, et al. Emission of volatile compounds from apple plants infested with Pandemis heparana larvae, antennal response of conspecific adults, and preliminary field trial[J]. Journal of Chemical Ecology, 2016, 42: 1265--1280. [28]Louis M, Dohet L, Grégoire J C. Fallen trees’ last stand against bark beetles[J]. Forest Ecology and Management, 2016, 359: 44--50.